Glossary entry (derived from question below)
English term or phrase:
(wash) stringency
Dutch translation:
stringentie (tijdens de wasprocedure)
Added to glossary by
Michiel Leeuwenburgh
Nov 2, 2011 13:39
12 yrs ago
3 viewers *
English term
Netherlands
PhD - Chemistry & pharma specialist
Native in: Dutch (Variant: Netherlands) 
Works in: English to Dutch
(wash) stringency
English to Dutch
Science
Biology (-tech,-chem,micro-)
moleculaire biologie, DNA-hydbridisatie
In een octrooitekst over DNA-constructen:
For example, hybridization of such sequences can be carried out under conditions of reduced stringency, medium stringency or even stringent conditions (e.g., conditions represented by a wash stringency of 35-40% formamide with 5x Denhardt’s solution, 0.5% SDS and 1x SSPE at 37°C; conditions represented by a wash stringency of 40-45% formamide with 5x Denhardt’s solution, 0.5% SDS, and 1x SSPE at 42°C; and conditions represented by a wash stringency of 50% formamide with 5x Denhardt’s solution, 0.5% SDS and 1x SSPE at 42°C, respectively).
Ik zie hier en daar dat *stringency* vertaald wordt met *striktheid*, maar ik ben er niet zeker van of dit in het vakgebied gangbaar is. Nog minder zeker ben ik van een vertaling van *wash stringency* (*wasstriktheid* bestaat in ieder geval niet...).
Alle suggesties zijn welkom!
For example, hybridization of such sequences can be carried out under conditions of reduced stringency, medium stringency or even stringent conditions (e.g., conditions represented by a wash stringency of 35-40% formamide with 5x Denhardt’s solution, 0.5% SDS and 1x SSPE at 37°C; conditions represented by a wash stringency of 40-45% formamide with 5x Denhardt’s solution, 0.5% SDS, and 1x SSPE at 42°C; and conditions represented by a wash stringency of 50% formamide with 5x Denhardt’s solution, 0.5% SDS and 1x SSPE at 42°C, respectively).
Ik zie hier en daar dat *stringency* vertaald wordt met *striktheid*, maar ik ben er niet zeker van of dit in het vakgebied gangbaar is. Nog minder zeker ben ik van een vertaling van *wash stringency* (*wasstriktheid* bestaat in ieder geval niet...).
Alle suggesties zijn welkom!
Proposed translations
(Dutch)
| 2 +3 | stringentie (tijdens de wasprocedure) |
Koen Speetjens (X)
|
| 3 | precizie |
Machteld/Johan Schrameijer/Westenburg
|
References
| definition |
Barend van Zadelhoff
|
Proposed translations
+3
16 mins
Selected
stringentie (tijdens de wasprocedure)
Niet echt veel referenties, maar laagstringente, lage stringentie en hoogstringente vind ik een paar keer (misschien het meeste in België)
4 KudoZ points awarded for this answer.
Comment: "Hartelijk bedankt, Koen!"
16 mins
Machteld/Johan Schrameijer/Westenburg
Native in English
&
Dutch
Machteld/Johan Schrameijer/Westenburg
over 35 years of US cultural experience
Native in: Dutch (Variant: Netherlands) 
, English (Variant: US)
Works in: English to Dutch, Dutch to English, Flemish to English, and 1 more.
precizie
Of graad van preciezie kan hier mischien goed passen.
(spelling?)
(spelling?)
Reference comments
32 mins
Barend van Zadelhoff
Netherlands
Netherlands
Native in Dutch
Netherlands
TR is the most intimate act of reading.
Native in: Dutch
Works in: English to Dutch, Dutch to English, Dutch
Related KudoZ points
:
32
Reference:
definition
Stringency - a term used in hybridization experiments to denote the degree of homology between the probe and the filter bound nucleic acid; the higher the stringency, the higher percent homology between the probe and filter bound nucleic acid
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The next step in a hybridization experiment is to wash the filter. This is normally done in two steps. First a non-stringent wash is performed to remove the non-specifically bound DNA and the second wash is performed at a higher stringency that only permits highly homologous sequences to remain bound to the filter. Controlling the stringency is an important step in these experiments
http://www.ndsu.edu/pubweb/~mcclean/plsc731/dna/dna6.htm
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The next step in a hybridization experiment is to wash the filter. This is normally done in two steps. First a non-stringent wash is performed to remove the non-specifically bound DNA and the second wash is performed at a higher stringency that only permits highly homologous sequences to remain bound to the filter. Controlling the stringency is an important step in these experiments
http://www.ndsu.edu/pubweb/~mcclean/plsc731/dna/dna6.htm
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